#Outline ##Introduction - Start with phenotypic plasticity? Or go right into epigenetics and DNA methylation? - DNA methylation in general - DNA methylation in invertebrates: gene body methylation - functions, potential role in phenotypic plasticity - DNA methylation in corals and why it is of interest: potential basis of phenotypic plasticity/acclimatization/resilience to env change - Introduce objectives of the study: explore patterns of germline methylation in coral transcriptomes and env response genes ##Methods - Description of datasets, including table - Description of methods used to annotate transcriptomes - Description of methods used to calculate CpG and analyze graphically/statistically ##Results - For each species, compare CpG O/E of whole-transcriptome to env response genes. - For each species, show density plot with env response gene profile superimposed over transcriptome CpG O/E - Compare density profiles statistically using some kind of bootstrapping technique - For each species, show bar plot of CpG O/E by GOslim bin for both whole transcriptome and env response genes - Show summary plot with ranking of GOslim bin by mean CpG O/E ###Discussion - This content will become more clear once results are complete, however likely topics include hypotheses for patterns of germline methylation in transcriptomes and env response genes.