FastQCFastQC Report
Wed 25 Nov 2015
D1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74550563
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA7253690.9729892985516421No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT3230780.43336761923581985No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG2518220.3377868521261201No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT2478850.3325058725579309No Hit
TGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTT1906280.25570296497962064No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAATTTCAC1584670.21256311639122027No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC1297270.17401209968058862No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA1277490.17135886686730992No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1102870.14793583785544315No Hit
GTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAA1081200.14502908582997556No Hit
CTTTAGTACCTTTTGCATAAGGGTTTTTCAAGATAAATTTAAGAATTTAA1076690.14442412728660414No Hit
ATTTTTTTGAGTTAAGAAATCGTGGTAGAGATTTGATTAAACTAAAGAAT1040770.1396059208835217No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA988430.13258518248882978No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA983120.1318729142260133No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAATTTCACC973510.1305838562211797No Hit
ATTTATTTAAAAATAAGCCTATGATCGGAGGGTCAGTGATTATGATAAAA964170.12933101524665883No Hit
AGAAACCAAAGATTTTTTAAATATCAATTTCACCATTTTTCAGCTTAACC956270.12827133176713904No Hit
ATAAAAACGAGTAAAAATATTATTTTCTTGGTATCACTGTTTTGTTTTAC949680.12738736795321048No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA910380.12211577798547275No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG903790.12123181417154422No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA885220.11874088730892615No Hit
TTTCAAGATAAATTTAAGAATTTAATTTTCCCGAATAAAAGAGAGTTATT884370.1186268707320158No Hit
CTTCAATTGAAAAGCAAATTATCGCGCTACCTTAGGACGCTCACGTTAAC838740.11250619260916915No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAA834030.11187440663593647No Hit
CTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTAAATTTTT824870.11064571034829072No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACC813210.10908167118737921No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT803010.10771347226445492No Hit
GAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTG797470.10697035245729801No Hit
AGAAACCAAAGATTTTTTAAACATCAATTTCACCATTTTTCAGCTTAACC783400.1050830427665583No Hit
CTTTCATACCATCCTTCAATTGAAAAGCAAATTATCGCGCTACCTTAGGA774250.10385568785040564No Hit
TAGAAACCAAAGATTTTTTAAATATCAATTTCACCATTTTTCAGCTTAAC767990.10301598929574818No Hit
CACCATTTTTCAGCTTAACCCAAAAAATTTTACCCCTTAAATAGTTAAAG759840.1019227715289018No Hit
GTTTAAAGCCTATAGAAACCAAAGATTTTTTAAATATCAATTTCACCATT753570.10108173160275127No Hit
GCACCTTCTTCGTTAACCCTTTCATACCATCCTTCAATTGAAAAGCAAAT747210.10022861933316318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT105700.040.1998861
TGTGAAA1948350.039.8854835
CTCCCGT95800.036.9535181
TTGTGAA2100150.035.9806674
GTGAAAA2273200.034.2776076
AGACGTG190400.033.476414
CCCGTCA98950.033.2412873
AAAAGTG2487150.031.4607359
CGTGCAT201750.030.8862887
GAAAAGT2562450.030.3026928
CGCGCTG8650.029.7047581
CGATTAC137100.027.9777724
CGGTGGG27050.026.0341131
TCGAGTC210950.026.0249542
CGGGGCA22150.024.9191171
CGGGCAT28950.024.6542071
TCGCGCA27600.024.627172
CCGGGAG156650.024.6038441
TTTTGTG3053500.024.5311722
TTTGTGA3003350.024.2539273
Produced by FastQC (version 0.11.2)