FastQCFastQC Report
Wed 25 Nov 2015
D4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53285111
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA5518991.0357471151744433No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA2902790.5447656851085475No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA2752650.5165889585929548No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT2607110.4892755126286591No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT2136690.40099193938058986No Hit
CTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAG1519950.2852485378138745No Hit
TGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTT1274450.2391756301305256No Hit
GTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCT1207140.2265435836288302No Hit
CTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTAAATTAAATTTTT1193000.22388993428201737No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA1018700.19117910817526496No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG973050.18261198705206788No Hit
ATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCC957810.17975190105168404No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA948810.178062873886103No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT921490.1729357380901393No Hit
GTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGAAACCAA917200.17213063514121232No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA878540.16487532511661654No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA872970.16383000497080696No Hit
GTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAA779380.146265999145615No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC749560.14066968913698988No Hit
TTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTA747820.14034314388497754No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC746860.14016298098731556No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTTTTGG746590.14011231017234815No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA699820.13133499900187878No Hit
CTTCACCTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTT677700.12718374556825077No Hit
CCCAATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCC658080.12350166634728414No Hit
TTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGAA641020.12030002152008279No Hit
AATTAATCTTTTTCTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAA604310.11341066738136288No Hit
GTCAGGGTGAGATACTCCTCTTTAGCGTTTAGTATAACTTTATAAAACAG578340.1085368856602363No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA549920.10320331321070157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT66150.043.2352451
TGTGAAA1649600.041.989555
TTGTGAA1745150.039.8401534
GTGAAAA1861650.037.209036
GAAAAGT2089450.032.6749158
AAAAGTG1885500.032.549189
TTTGTGA2198500.031.7896543
CCCGTCA79400.031.6522983
TTTTGTG2296000.031.2255332
CGGAGGG806350.031.1676031
GGAGGGT820350.030.695112
CGCGCAC22950.030.2231883
TCGCGCA23800.029.9437922
GGGTTGC212700.029.2797785
AGACGTG127000.028.6146934
CGGGGCA21250.027.9461751
CGTGCAT130500.027.4840057
CCGGAGG60100.027.1138341
GAGGGTT231450.026.212223
GTTTTGT3037200.026.1740951
Produced by FastQC (version 0.11.2)