FastQCFastQC Report
Wed 25 Nov 2015
D5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65005727
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA3235990.4978007553088361No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC2196770.33793484072564867No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG1723440.26512125616255316No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT1658870.25518828517985803No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT1629740.2507071415415445No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1328890.20442660382830577No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA1308670.20131610865608812No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA1293430.19897169983192406No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA1223190.1881664980071679No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACC1140660.17547069352827943No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA921910.14181981227592455No Hit
CTGAATCATACACATAAGACCAATATCTACTTTTGATCCAATATGAAGGC910600.1400799655698028No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA857140.13185607477322728No Hit
TTTTAAATGAACTCGGCAAACTAAACTTCTCGACTGTTTAACAAAAACAT855410.13158994437520866No Hit
CACAGATTTGGGACAAAGCATTGCTTTAGCGAAAAAGTCCTTTCGTACAA845620.13008392322110326No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA844320.12988394084108928No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA836060.12861328356500035No Hit
GTCTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTT827630.1273164747469096No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA811460.12482900160473553No Hit
TAGAAACCAAAGATTTTTTAAACATCAATTTCACCATTTTTCAGCTTAAC789080.12138622801649461No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA770150.11847417689829082No Hit
GTTTACCTTAGTTCAATATCTACATCTTTAAGATCCAATATGAAGTTAGC767630.11808651874626369No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT733230.11279467730589338No Hit
ATAAAAACGAGTAAAAATATTATTTTCTTGGTATCACTGTTTTGTTTTAC726630.11177938214582232No Hit
CGGCAAACTAAACTTCTCGACTGTTTAACAAAAACATTTCCTTTTGATGT718880.1105871794957389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT92700.058.9419141
CGCGCTG10750.044.717241
TGGCCCG48350.039.4900558
GGCCCGG53250.036.213019
CGGAGTC119500.035.0889241
TCGAGTG59550.034.8544247
CGCCTAT107100.034.7519651
CCGTCAA158900.034.339664
CGGGGCA15200.034.1306841
CCCGTCA155800.033.9247253
AGACGTG158050.032.780084
CGACATG97050.031.9813393
TCCCGTC165400.031.7548032
CGTGCAT161500.031.0268847
TGTGAAA1588950.030.6388055
GTGAAAA1638550.029.7403356
CGTCAAA186100.029.6563685
ACCCCTA207500.029.11578
GTGCGCG15750.028.9500228
CGGGCAT22100.028.6431921
Produced by FastQC (version 0.11.2)