FastQCFastQC Report
Wed 25 Nov 2015
D5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65005727
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA3543470.5451012031601462No Hit
GCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCAC2128350.32740961423291215No Hit
CTCCCATCAAAATCGTATAAGAGCCTGAAAGCTCCAATTAAGATAAAGCT1966120.3024533515331657No Hit
CGGAGGGTCAGTGATTATGATAAAAACGAGTAAAAATATTATTTTCTTGG1685010.25920946934413946No Hit
CTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTTTGCATAAGGGTT1680170.2584649195600874No Hit
GTTTTGTGAAAAGTAGACTTGAACGAAGCTAGTATAAATAAACTTTAGTA1431350.2201882920254088No Hit
GTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAAGCCTATAGA1349040.2075263307185227No Hit
CTCGTTTTAAAAAGAGGATAGAAACTGAACTAGCTCACGCCGTTCTGAAC1343510.2066756364404632No Hit
CTAATAGCCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAA1209180.18601130328101706No Hit
CCAGTTTAAAGCCTATAGAAACCAAAGATTTTTTAAACATCAATTTCACC1070380.16465933839952288No Hit
CTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTTTA1056750.16256260006137613No Hit
GTCTTGTCGTCCTTCTTTTACATAAAGGCCTCTTCACCTTAAAGTTAGTT985220.1515589541826061No Hit
CTCCGTTTGGTTTTAGTTAAAATACACTAATGCCTAATAGCCAGTTTAAA954480.14683014005827516No Hit
CTTAAAGTTAGTTTAAATTAAATTTTTAGACACAGCACCTTCTTCGTTAA892600.13731097876960902No Hit
CACAGATTTGGGACAAAGCATTGCTTTAGCGAAAAAGTCCTTTCGTACAA858640.13208682367324345No Hit
CTGAATCATACACATAAGACCAATATCTACTTTTGATCCAATATGAAGGC808640.12439519367270517No Hit
CTCGTTTTTATCATAATCACTGACCCTCCGATCATAGGCTTATTTTTAAA790060.12153698396450516No Hit
GTTTACCTTAGTTCAATATCTACATCTTTAAGATCCAATATGAAGTTAGC785560.12084473726445671No Hit
CCAACATCGAGGTCGCAATCTTCCCCTCCAATACCATCTTTCGGGAGAAA783650.12055091699843615No Hit
TTTTAAATGAACTCGGCAAACTAAACTTCTCGACTGTTTAACAAAAACAT752780.11580210463610383No Hit
GTGAAAAGTGGACTTGAACGAAGCTAGTATAAATAAACTTTAGTACCTTT726800.11180553368782414No Hit
TAGAAACCAAAGATTTTTTAAACATCAATTTCACCATTTTTCAGCTTAAC709280.10911038653563555No Hit
GTTTTGTTTTACCTTAAAAGATTGATGGCTAAATAAAGGTTTTTAAATGA706400.10866734864760455No Hit
CTTCTCTTTACATTCCAGTTTCACCTTGTTCGGTTTCTCCTTTCTGCATG676350.10404467901728104No Hit
GTTTTTTCTAGATTTGATGCTCACAGCTATAACTTAATCTGGCTATCTAT655090.10077419794105218No Hit
CGGCAAACTAAACTTCTCGACTGTTTAACAAAAACATTTCCTTTTGATGT651660.10024655212301525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT110300.064.004391
CGCGCTG9750.046.777071
TGGCCCG51700.041.901518
CCGTCAA171300.040.0163964
CCCGTCA177750.038.7809873
CGCCTAT109300.038.206311
TCCCGTC187550.036.427522
CGTCAAA197700.034.816895
TCGAGTG56900.034.397247
GGCCCGG63500.034.2602849
CGGAGTC124050.034.0464171
AGACGTG150850.033.3487554
CGGGGCA16700.033.2840231
GTGCGCG16300.032.3512278
TGTGAAA1608800.032.1111265
TCGTGCG15150.031.9835177
GTGAAAA1658850.031.1248136
CGACATG89850.031.0899723
CGTGCAT162400.030.1878597
CGCTCGT24050.029.235671
Produced by FastQC (version 0.11.2)