**Sequence Assembly**

Raw sequence reads were quality trimmed using trim galore (citation) [code/output](https://github.com/sr320/paper-pano-go/wiki/Quality-trim-output), and quality assessed using FastQC (citation). Assembly of sequence reads was carried out using Trinity (citation) using the following parameters...

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Raw sequence reads were quality trimmed using trim galore (Krueger 2013), and quality assessed using FastQC (Andrews 2010) eliminating adapters and with a 20 quality Phred score cutoff (Ewing et al. 1998[not sure about this reference]). Assembly of sequence reads was carried out using Trinity (Grabherr et al. 2011) with default parameters.