This workflow will take focus on taking a simple SQLShare table that has gene IDs and associated expression data and will take you through the steps of figuring out the name, function, etc of each gene. --- ### Initial Data Table: Oyster larvae RNA-seq - OsHV exposure SCREENSHOT SQLShare%20-%20View%20Query --- --- ### Join with [qDOD Cgigas Gene Descriptions Swiss-prot](https://sqlshare.escience.washington.edu/sqlshare#s=query/sr320%40washington.edu/qDOD%20Cgigas%20Gene%20Descriptions%20(Swiss-prot)) Select * from [sr320@washington.edu].[solid0078_20091105_RobertsLab_GE_F3 trimmed RNA-Seq.txt]oshv left join [sr320@washington.edu].[qDOD Cgigas Gene Descriptions (Swiss-prot)]des on oshv.ID = des.CGI_ID ​ --- ### Identifying Expressed Genes Enriched in the Dataset ``` Select * from [sr320@washington.edu].[solid0078_20091105_RobertsLab_GE_F3 trimmed RNA-Seq.txt]oshv left join [sr320@washington.edu].[qDOD Cgigas Gene Descriptions (Swiss-prot)]des on oshv.ID = des.CGI_ID ​where UniqueReads >=10​ ``` --- Download file. Corresponding non-redundant SPIDs @ --- ### Joining with GO information (option 1) Joining with SELECT * FROM [sr320@washington.edu].[Cgigas Larvae RNA-Seq OsHV UR10]ur left join [sr320@washington.edu].[qDOD_Cgigas_GO_GOslim_DISTINCT]ds on ur.ID = ds.CGI_ID ​ SELECT DISTINCT ID, SPID1, GOID, term, aspect FROM [sr320@washington.edu].[Cgigas Larvae RNA-Seq OsHV UR10]ur left join [sr320@washington.edu].[qDOD_Cgigas_GO_GOslim_DISTINCT]ds on ur.ID = ds.CGI_ID ​