Left read files: $VAR1 = [
          '/gscratch/srlab/sr320/data/NR021_R1_comb.fastq'
        ];
Right read files: $VAR1 = [
          '/gscratch/srlab/sr320/data/NR021_R1_comb.fastq'
        ];
Trinity version: Trinity-v2.4.0
-ERROR: couldn't run the network check to confirm latest Trinity software version.

Friday, August 4, 2017: 10:18:33	CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /gscratch/srlab/programs/trinity/util/support_scripts/ExitTester.jar 0
Friday, August 4, 2017: 10:18:33	CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /gscratch/srlab/programs/trinity/util/support_scripts/ExitTester.jar 1
Friday, August 4, 2017: 10:18:33	CMD: mkdir -p /gscratch/srlab/sr320/analyses/trinity_out_dir
Friday, August 4, 2017: 10:18:33	CMD: mkdir -p /gscratch/srlab/sr320/analyses/trinity_out_dir/chrysalis


----------------------------------------------------------------------------------
-------------- Trinity Phase 1: Clustering of RNA-Seq Reads  ---------------------
----------------------------------------------------------------------------------

---------------------------------------------------------------
------ Quality Trimming Via Trimmomatic  ---------------------
<< ILLUMINACLIP:/gscratch/srlab/programs/trinity/trinity-plugins/Trimmomatic/adapters/TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25 >>
---------------------------------------------------------------


## Running Trimmomatic on read files: /gscratch/srlab/sr320/data/NR021_R1_comb.fastq, /gscratch/srlab/sr320/data/NR021_R1_comb.fastq
Friday, August 4, 2017: 10:18:33	CMD: java -jar /gscratch/srlab/programs/trinity/trinity-plugins/Trimmomatic/trimmomatic.jar PE -threads 24 -phred33  /gscratch/srlab/sr320/data/NR021_R1_comb.fastq /gscratch/srlab/sr320/data/NR021_R1_comb.fastq  NR021_R1_comb.fastq.P.qtrim NR021_R1_comb.fastq.U.qtrim  NR021_R1_comb.fastq.P.qtrim NR021_R1_comb.fastq.U.qtrim  ILLUMINACLIP:/gscratch/srlab/programs/trinity/trinity-plugins/Trimmomatic/adapters/TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25 
TrimmomaticPE: Started with arguments:
 -threads 24 -phred33 /gscratch/srlab/sr320/data/NR021_R1_comb.fastq /gscratch/srlab/sr320/data/NR021_R1_comb.fastq NR021_R1_comb.fastq.P.qtrim NR021_R1_comb.fastq.U.qtrim NR021_R1_comb.fastq.P.qtrim NR021_R1_comb.fastq.U.qtrim ILLUMINACLIP:/gscratch/srlab/programs/trinity/trinity-plugins/Trimmomatic/adapters/TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
slurmstepd: error: Job 54792 exceeded memory limit (10594664 > 512000), being killed
slurmstepd: error: Exceeded job memory limit
slurmstepd: error: *** JOB 54792 ON n2158 CANCELLED AT 2017-08-04T10:18:53 ***