Left read files: $VAR1 = [ '/gscratch/srlab/sr320/data/NR021_R1_comb.fastq' ]; Right read files: $VAR1 = [ '/gscratch/srlab/sr320/data/NR021_R2_comb.fastq' ]; Trinity version: Trinity-v2.4.0 -ERROR: couldn't run the network check to confirm latest Trinity software version. Friday, August 4, 2017: 10:48:33 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /gscratch/srlab/programs/trinity/util/support_scripts/ExitTester.jar 0 Friday, August 4, 2017: 10:48:33 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /gscratch/srlab/programs/trinity/util/support_scripts/ExitTester.jar 1 ---------------------------------------------------------------------------------- -------------- Trinity Phase 1: Clustering of RNA-Seq Reads --------------------- ---------------------------------------------------------------------------------- --------------------------------------------------------------- ------ Quality Trimming Via Trimmomatic --------------------- << ILLUMINACLIP:/gscratch/srlab/programs/trinity/trinity-plugins/Trimmomatic/adapters/TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25 >> --------------------------------------------------------------- ## Running Trimmomatic on read files: /gscratch/srlab/sr320/data/NR021_R1_comb.fastq, /gscratch/srlab/sr320/data/NR021_R2_comb.fastq ############################################################################### #### Trimmomatic process was previously completed. Skipping it and using existing qual-trimmed files: NR021_R1_comb.fastq.PwU.qtrim.fq, NR021_R2_comb.fastq.PwU.qtrim.fq ############################################################################### --------------------------------------------------------------- ------------ In silico Read Normalization --------------------- -- (Removing Excess Reads Beyond 50 Coverage -- --------------------------------------------------------------- # running normalization on reads: $VAR1 = [ [ 'NR021_R1_comb.fastq.PwU.qtrim.fq' ], [ 'NR021_R2_comb.fastq.PwU.qtrim.fq' ] ]; Friday, August 4, 2017: 10:48:34 CMD: /gscratch/srlab/programs/trinity/util/insilico_read_normalization.pl --seqType fq --JM 500G --max_cov 50 --CPU 50 --output /gscratch/srlab/sr320/analyses/trinity_out_dir/insilico_read_normalization --max_pct_stdev 10000 --left NR021_R1_comb.fastq.PwU.qtrim.fq --right NR021_R2_comb.fastq.PwU.qtrim.fq --pairs_together --PARALLEL_STATS Converting input files. (both directions in parallel)left file exists, nothing to doright file exists, nothing to do------------------------------------------- ----------- Jellyfish -------------------- -- (building a k-mer catalog from reads) -- ------------------------------------------- -sorting each stats file by read name. Done converting input files.Thread 4 terminated abnormally: Error, not all specified records have been retrieved (missing 907086) from /gscratch/srlab/sr320/analyses/trinity_out_dir/NR021_R2_comb.fastq.PwU.qtrim.fq, see file: /gscratch/srlab/sr320/analyses/trinity_out_dir/insilico_read_normalization/NR021_R2_comb.fastq.PwU.qtrim.fq.normalized_K25_C50_pctSD10000.fq.missing_accs for list of missing entries at /gscratch/srlab/programs/trinity/util/insilico_read_normalization.pl line 548. Thread 3 terminated abnormally: Error, not all specified records have been retrieved (missing 907086) from /gscratch/srlab/sr320/analyses/trinity_out_dir/NR021_R1_comb.fastq.PwU.qtrim.fq, see file: /gscratch/srlab/sr320/analyses/trinity_out_dir/insilico_read_normalization/NR021_R1_comb.fastq.PwU.qtrim.fq.normalized_K25_C50_pctSD10000.fq.missing_accs for list of missing entries at /gscratch/srlab/programs/trinity/util/insilico_read_normalization.pl line 548. Error encountered with thread. Error encountered with thread. Error, at least one thread died at /gscratch/srlab/programs/trinity/util/insilico_read_normalization.pl line 419. Error, cmd: /gscratch/srlab/programs/trinity/util/insilico_read_normalization.pl --seqType fq --JM 500G --max_cov 50 --CPU 50 --output /gscratch/srlab/sr320/analyses/trinity_out_dir/insilico_read_normalization --max_pct_stdev 10000 --left NR021_R1_comb.fastq.PwU.qtrim.fq --right NR021_R2_comb.fastq.PwU.qtrim.fq --pairs_together --PARALLEL_STATS died with ret 6400 at /gscratch/srlab/programs/trinity/Trinity line 2462.