Left read files: $VAR1 = [ '/gscratch/srlab/sr320/data/NR021_S8_L001_R1_001.fastq', '', '/gscratch/srlab/sr320/data/NR021_S8_L002_R1_001.fastq' ]; Right read files: $VAR1 = [ '/gscratch/srlab/sr320/data/NR021_S8_L001_R2_001.fastq', '', '/gscratch/srlab/sr320/data/NR021_S8_L002_R2_001.fastq' ]; Trinity version: Trinity-v2.4.0 -ERROR: couldn't run the network check to confirm latest Trinity software version. Friday, August 4, 2017: 10:58:34 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /gscratch/srlab/programs/trinity/util/support_scripts/ExitTester.jar 0 Friday, August 4, 2017: 10:58:34 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /gscratch/srlab/programs/trinity/util/support_scripts/ExitTester.jar 1 Error, cannot locate file: at /gscratch/srlab/programs/trinity/Trinity line 2429. main::create_full_path('ARRAY(0xa784d8)', 1) called at /gscratch/srlab/programs/trinity/Trinity line 1159