FastQCFastQC Report
Wed 24 Jan 2018
Geoduck-5_S1_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-5_S1_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305913830
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT840000.048.013726
ACGACGC889000.045.864765
GACGCTC937850.042.7182127
CACGACG1003500.040.552094
CGCTCTT995300.040.5146989
ACGCTCT1117500.035.9286588
CTACACG1451450.028.9164091
TACACGA2069750.020.3548812
ACACGAC2128400.019.7667353
GCTCTTC1295550.06.861879310-14
TCTTCCG1530850.06.306335410-14
CTTCCGA1696700.06.073613610-14
CCCTAAC12326250.05.7993793
ACCCTAA12705150.05.7810682
TAACCCT12703550.05.48046546
AACCCTA13314950.05.46521571
CCTAACC13290600.05.43149664
CTAACCC12984950.05.31479645
CCGATCT2119100.05.057342515-19