Basic Statistics
Measure | Value |
---|---|
Filename | 10_32_S32_L001_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 574346 |
Sequences flagged as poor quality | 0 |
Sequence length | 35-76 |
%GC | 25 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 6118 | 1.0652115623683285 | TruSeq Adapter, Index 7 (97% over 38bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT | 1124 | 0.19570084931382825 | TruSeq Adapter, Index 7 (97% over 38bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 775 | 0.0 | 63.04847 | 1 |
GTCTGAA | 805 | 0.0 | 60.268353 | 17 |
CTGCTTG | 800 | 0.0 | 60.219646 | 59 |
TGCTTGA | 795 | 0.0 | 60.201565 | 60 |
AGAGCAC | 815 | 0.0 | 59.954067 | 8 |
CGTCTGA | 810 | 0.0 | 59.896328 | 16 |
TCTGCTT | 805 | 0.0 | 59.84561 | 58 |
TCTGAAC | 805 | 0.0 | 59.837864 | 18 |
TGAACTC | 805 | 0.0 | 59.837864 | 20 |
CTCCAGT | 795 | 0.0 | 59.718735 | 24 |
AAGAGCA | 825 | 0.0 | 59.647408 | 7 |
GCACACG | 820 | 0.0 | 59.588493 | 11 |
ACGTCTG | 815 | 0.0 | 59.528862 | 15 |
CTGAACT | 810 | 0.0 | 59.468494 | 19 |
TTCTGCT | 810 | 0.0 | 59.429222 | 57 |
GCTTGAA | 795 | 0.0 | 59.348785 | 61 |
AGTCACG | 800 | 0.0 | 59.34549 | 28 |
TCCAGTC | 800 | 0.0 | 59.34549 | 25 |
CAGTCAC | 800 | 0.0 | 59.34549 | 27 |
ACTCCAG | 800 | 0.0 | 59.34549 | 23 |