FastQCFastQC Report
Wed 14 Mar 2018
17_9_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename17_9_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403861
Sequences flagged as poor quality0
Sequence length35-76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA99122.454309774897799Illumina Single End PCR Primer 1 (97% over 34bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9220.22829636929537636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT12850.068.5925262
CGTATCA12650.068.3256161
GCCGTAT12450.067.83015459
GATCGGA13950.067.260211
CCGTATC12600.067.2571560
CGCCGTA12850.067.1829458
TAGGCTA13450.067.0098832
TAGATCT13650.066.5737444
TCTCGGT13150.066.3758648
ATCTCGG13400.066.359847
AGCGTCG13800.066.254310
GGTCGCC13200.066.2525455
TCGGTGG13300.066.0802650
GGCTATA13500.066.0575834
CGTCGTG13850.066.0151212
GTAGGCT13650.065.9952631
AGATCTC13500.065.8767645
GCTATAG13650.065.8505935
GAAGAGC14200.065.83496
GAGCGTC13850.065.7678769
Produced by FastQC (version 0.11.5)