Basic Statistics
Measure | Value |
---|---|
Filename | 19_7_S7_L001_R2_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 371667 |
Sequences flagged as poor quality | 0 |
Sequence length | 35-76 |
%GC | 21 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA | 813 | 0.21874419843569648 | Illumina Single End PCR Primer 1 (96% over 33bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTAT | 65 | 0.0 | 69.71728 | 59 |
CGCCGTA | 65 | 0.0 | 69.67959 | 58 |
TCGCCGT | 65 | 0.0 | 69.65136 | 57 |
GTCGCCG | 70 | 0.0 | 69.585556 | 56 |
GGTCGCC | 70 | 0.0 | 69.557396 | 55 |
CTCGGTG | 75 | 0.0 | 69.4637 | 49 |
TCTCGGT | 75 | 0.0 | 69.444984 | 48 |
ATCTCGG | 75 | 0.0 | 69.41694 | 47 |
GAGCGTC | 75 | 0.0 | 69.37958 | 9 |
AAGAGCG | 75 | 0.0 | 69.37958 | 7 |
GAAGAGC | 75 | 0.0 | 69.37958 | 6 |
GTCCTGG | 75 | 0.0 | 69.37958 | 35 |
GCGTCGT | 75 | 0.0 | 69.37958 | 11 |
CGTCGTG | 75 | 0.0 | 69.37958 | 12 |
CTGGTGT | 70 | 0.0 | 69.37957 | 38 |
CGTCCTG | 80 | 0.0 | 69.37957 | 34 |
TCGGTGG | 80 | 0.0 | 65.13098 | 50 |
GATCTCG | 80 | 0.0 | 65.07838 | 46 |
AGATCTC | 80 | 0.0 | 65.06961 | 45 |
TAGATCT | 80 | 0.0 | 65.06961 | 44 |