FastQCFastQC Report
Wed 14 Mar 2018
1_25_S25_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1_25_S25_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488137
Sequences flagged as poor quality0
Sequence length35-76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA369337.5661136115475776Illumina Single End PCR Primer 1 (97% over 34bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11270.23087780684520942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT41750.068.731159
CGTATCA42050.068.6158461
CGCCGTA42300.068.437358
CCGTATC41850.068.4009160
GTCGCCG42950.068.3351856
GATCGGA44900.068.2220841
AGATCTC43900.068.2164345
GATCTCG43900.068.1725446
ATCGGAA45100.068.1492
TCGGAAG45000.068.147133
TCTCGGT43750.068.1288648
CTCGGTG43550.068.0913649
GCGTCGT44450.068.059111
CGGAAGA45150.067.997134
GAGCGTC44750.067.988269
GGTCGCC43100.067.966555
GTATCAT42550.067.8807262
TGGTCGC43200.067.8651554
GTAGATC44500.067.8440543
AGCGTCG44800.067.8353710
Produced by FastQC (version 0.11.5)