FastQCFastQC Report
Wed 14 Mar 2018
9_31_S31_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename9_31_S31_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595446
Sequences flagged as poor quality0
Sequence length35-76
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA22540.3785397836243757TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC206.9133093E-493.9469270
GTATGCC3000.068.2876847
AGCACAC3050.068.1976310
AAGAGCA3050.068.197637
GAGCACA3050.068.197639
TGCTTGA3000.067.54513560
TTCTGCT3100.067.45466657
TCTGCTT3050.067.4519858
TATGCCG3050.067.18516548
CGTATGC3050.067.15690646
GAAGAGC3150.067.133176
TGAACTC3100.067.09766420
GCACACG3100.067.09766411
ACGTCTG3100.067.09766415
AGAGCAC3100.067.0976648
GCTTGAA3050.066.4997761
CTGCTTG3100.066.42016659
TCTTCTG3150.066.32793455
TGCCGTC3100.066.1627950
AGATTCC3150.066.04372435
Produced by FastQC (version 0.11.5)