Bismark report for: /Volumes/Serine/wd/18-03-16/10_32_S32_L001_R1_001.fastq.gz and /Volumes/Serine/wd/18-03-16/10_32_S32_L001_R2_001.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.6 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 574346 Number of paired-end alignments with a unique best hit: 230846 Mapping efficiency: 40.2% Sequence pairs with no alignments under any condition: 262321 Sequence pairs did not map uniquely: 81179 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 37825 ((converted) top strand) GA/CT/CT: 77060 (complementary to (converted) top strand) GA/CT/GA: 78343 (complementary to (converted) bottom strand) CT/GA/GA: 37618 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 6729925 Total methylated C's in CpG context: 689306 Total methylated C's in CHG context: 15135 Total methylated C's in CHH context: 47064 Total methylated C's in Unknown context: 2799 Total unmethylated C's in CpG context: 258687 Total unmethylated C's in CHG context: 1469826 Total unmethylated C's in CHH context: 4249907 Total unmethylated C's in Unknown context: 19823 C methylated in CpG context: 72.7% C methylated in CHG context: 1.0% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 12.4% Bismark completed in 0d 0h 4m 43s