Bismark report for: /Volumes/Serine/wd/18-04-07/zr2096_4_s1_R1.fastq.gz and /Volumes/Serine/wd/18-04-07/zr2096_4_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 24341968 Number of paired-end alignments with a unique best hit: 4516691 Mapping efficiency: 18.6% Sequence pairs with no alignments under any condition: 18136564 Sequence pairs did not map uniquely: 1688713 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2251307 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 2265382 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 148447494 Total methylated C's in CpG context: 14022606 Total methylated C's in CHG context: 1147944 Total methylated C's in CHH context: 4897777 Total methylated C's in Unknown context: 205137 Total unmethylated C's in CpG context: 5922512 Total unmethylated C's in CHG context: 33264805 Total unmethylated C's in CHH context: 89191850 Total unmethylated C's in Unknown context: 643585 C methylated in CpG context: 70.3% C methylated in CHG context: 3.3% C methylated in CHH context: 5.2% C methylated in unknown context (CN or CHN): 24.2% Bismark completed in 0d 1h 44m 39s