Bismark report for: /Volumes/Serine/wd/18-04-07/zr2096_5_s1_R1.fastq.gz and /Volumes/Serine/wd/18-04-07/zr2096_5_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 31778715 Number of paired-end alignments with a unique best hit: 5499890 Mapping efficiency: 17.3% Sequence pairs with no alignments under any condition: 24300069 Sequence pairs did not map uniquely: 1978756 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2749961 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 2749928 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 189151053 Total methylated C's in CpG context: 17864954 Total methylated C's in CHG context: 1429272 Total methylated C's in CHH context: 5654793 Total methylated C's in Unknown context: 253526 Total unmethylated C's in CpG context: 7027555 Total unmethylated C's in CHG context: 41425253 Total unmethylated C's in CHH context: 115749226 Total unmethylated C's in Unknown context: 809323 C methylated in CpG context: 71.8% C methylated in CHG context: 3.3% C methylated in CHH context: 4.7% C methylated in unknown context (CN or CHN): 23.9% Bismark completed in 0d 2h 14m 53s