Bismark report for: /Volumes/Serine/wd/18-04-07/zr2096_8_s1_R1.fastq.gz and /Volumes/Serine/wd/18-04-07/zr2096_8_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 29761837 Number of paired-end alignments with a unique best hit: 4646143 Mapping efficiency: 15.6% Sequence pairs with no alignments under any condition: 23487537 Sequence pairs did not map uniquely: 1628157 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2325979 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 2320162 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 157822868 Total methylated C's in CpG context: 14123725 Total methylated C's in CHG context: 1232277 Total methylated C's in CHH context: 5100411 Total methylated C's in Unknown context: 204421 Total unmethylated C's in CpG context: 5895006 Total unmethylated C's in CHG context: 35229169 Total unmethylated C's in CHH context: 96242280 Total unmethylated C's in Unknown context: 663867 C methylated in CpG context: 70.6% C methylated in CHG context: 3.4% C methylated in CHH context: 5.0% C methylated in unknown context (CN or CHN): 23.5% Bismark completed in 0d 1h 58m 10s