Bismark report for: /Volumes/Serine/wd/18-04-07/zr2096_9_s1_R1.fastq.gz and /Volumes/Serine/wd/18-04-07/zr2096_9_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 32636231 Number of paired-end alignments with a unique best hit: 5644532 Mapping efficiency: 17.3% Sequence pairs with no alignments under any condition: 24857593 Sequence pairs did not map uniquely: 2134106 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2816231 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 2828300 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 203684656 Total methylated C's in CpG context: 23798172 Total methylated C's in CHG context: 1639976 Total methylated C's in CHH context: 5453380 Total methylated C's in Unknown context: 272146 Total unmethylated C's in CpG context: 6006825 Total unmethylated C's in CHG context: 45798164 Total unmethylated C's in CHH context: 120988139 Total unmethylated C's in Unknown context: 826171 C methylated in CpG context: 79.8% C methylated in CHG context: 3.5% C methylated in CHH context: 4.3% C methylated in unknown context (CN or CHN): 24.8% Bismark completed in 0d 2h 19m 33s