FastQCFastQC Report
Sat 7 Apr 2018
zr2096_10_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_10_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17717127
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT10050.080.419561
CCTGGCT1900.078.7735441
TGCTGCT4650.075.409631
GGCTGCT3750.075.26251
CAGTGCT4200.074.325781
GCCAGCT2800.071.780381
GCCTGAG900.071.27131
CTGTGCT6150.069.5329741
GCCATGG501.6552804E-1068.420451
GGCCCAC258.8808316E-568.420451
TGTGCTC25450.067.1872942
GCTGGCT2550.067.078871
TTGGCAC2250.066.495823
TGGCACC2000.066.2536544
CCGTGCT5650.065.0902561
GACTGCT2250.064.6193161
GCCTGGT1600.064.144171
GCCAGGT200.003146451264.144171
TGCAGCT1400.064.144171
GGCACCC2150.063.606655
Produced by FastQC (version 0.11.5)