FastQCFastQC Report
Sat 7 Apr 2018
zr2096_1_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_1_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28982766
Sequences flagged as poor quality0
Sequence length20-100
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT8100.080.760231
GCCTGCT25100.080.570971
TGCAGCT6300.079.4029161
CCTGGCT9550.078.795321
CCAGGCT7050.077.626861
GCCTGGG5150.077.208821
GTCTGCT7300.076.725481
GCCTGGA4000.075.890871
CCGTGCT10500.075.330981
CATGGCT6050.074.910321
ACTGGCT5450.074.5277941
CTAGGCT5700.073.509311
GCTAGCT4600.073.427781
CCCTGGG9100.072.825161
CTGTGCT8250.072.554651
GCATGCT2250.072.209161
GCCTGGT6000.071.971621
GCCTGAT7700.071.0739441
CCCTGAG4700.070.95581
GTCAGCT7450.070.5894851
Produced by FastQC (version 0.11.5)