FastQCFastQC Report
Sat 7 Apr 2018
zr2096_1_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_1_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28982766
Sequences flagged as poor quality0
Sequence length20-100
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT19750.079.2574161
GCCTGCT51700.077.7612461
CTGTGCT26600.076.372661
CCGTGCT25300.076.239841
CCTGGCT6050.074.933571
GTCTGCT15200.073.156971
GTGTGCT26600.070.584411
GGGTGCT15800.070.108161
CGCTGCT15250.068.710351
ACTGGCT5250.068.42991
GCCTGAT5000.068.429891
CTAGGCT4450.068.237681
CCAGGCT5900.068.1399461
GCCTGGG3100.067.602121
CCCTGCT69400.067.542481
GCGTGCT14250.067.529511
GCATGCT4400.067.069081
TGTGCTC103100.064.881992
GCCTGTA2250.064.6282351
CCTGGGA6350.064.619862
Produced by FastQC (version 0.11.5)