FastQCFastQC Report
Sat 7 Apr 2018
zr2096_2_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_2_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30798582
Sequences flagged as poor quality0
Sequence length20-100
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGCT5150.077.501771
CCTGGCT8400.075.353611
GCCTGCT16650.075.0638961
CCGTGCT6450.074.382521
GCCTGGT7450.074.1392061
GGCCCTA503.6379788E-1272.569841
GCCTGGG4750.072.1454541
GCCTGGA4450.071.573241
CAGTGCT5500.071.1037751
ACTGGCT6200.070.2288741
CATGGCT4600.070.1157761
CTAGGCT5350.070.0830151
GCCTGAT7650.069.565861
GCCTAGG3200.069.294121
CCAGGCT6750.068.687491
GTCAGCT5600.067.6742551
CTGTGCT6000.067.194291
GGCAGCT3350.066.191391
GCCTGTG2200.065.972581
GCCCGGT559.094947E-1265.972581
Produced by FastQC (version 0.11.5)