FastQCFastQC Report
Sat 7 Apr 2018
zr2096_3_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_3_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29892002
Sequences flagged as poor quality0
Sequence length20-100
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT15500.079.684421
GCCTGCT39650.079.310951
CTGTGCT21800.077.926661
CCGTGCT23100.077.0083851
CCTGGCT5550.074.4338761
GTCTGCT13350.073.571931
TGCAGCT3700.072.914811
CCAGGCT4750.072.7709051
CCCTGCT55100.070.154461
CGCTGCT11600.070.135321
GTGTGCT22800.069.886691
GCATGCT2900.068.318351
GCCTGGT4150.068.055661
GCCTGGG2850.068.0378341
GTCAGCT3600.067.914581
GGGTGCT12950.067.706611
TGTGCTC80400.066.601832
CAGGGCT1400.066.2418061
GCCTGGA2500.065.760051
ACTGGCT5400.065.572691
Produced by FastQC (version 0.11.5)