FastQCFastQC Report
Sat 7 Apr 2018
zr2096_4_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_4_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24341968
Sequences flagged as poor quality0
Sequence length20-100
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGCT6750.079.504561
CCTGGCT8400.079.493731
GCCTGCT13700.078.642941
CCAGGCT7350.076.358571
CATGGCT4950.075.31141
GCCTGGA2950.074.9887851
GCCCTTG1450.073.456441
GCCTGAT8150.073.3871151
CCGTGCT6400.072.9708561
GGCCTAT2250.072.828611
GCTAGCT4150.072.060841
GGCCACT403.07773E-971.690671
GCCTGGG3200.071.690671
CAGTGCT4350.071.5729451
GCCTGGT6150.071.274341
GCCTGGC750.071.00791
GTCAGCT5850.070.727781
CTAGGCT5100.070.686591
ACTGGCT6050.070.421061
GGCAGCT3150.070.2275851
Produced by FastQC (version 0.11.5)