FastQCFastQC Report
Sat 7 Apr 2018
zr2096_4_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_4_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24341968
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT10100.078.692751
GCCTGCT26750.075.658471
CCGTGCT16900.074.9076461
CTGTGCT17300.073.649311
GCCTGGG1600.071.695591
GTCTGCT8850.070.82761
GCCTGGA1850.068.65061
CCCTGCT38350.067.195411
CCTGGCT4150.067.1297761
GTGTGCT18100.066.093491
GCCTGAT4850.065.888141
GCCTGGT3900.065.130061
CGCTGCT10050.064.8164751
ACTGGCT4150.064.168171
GCATGCT2950.063.883721
GGGTGCT9850.063.6369821
CCAGGCT4450.063.5248261
TGCAGCT2650.063.3858571
ACGTGCT5600.062.1849441
GGCCCAA1000.061.4533581
Produced by FastQC (version 0.11.5)