FastQCFastQC Report
Sat 7 Apr 2018
zr2096_5_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_5_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31778715
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCT4350.078.8717351
CAGTGCT1750.077.452881
GCCTGCT6600.077.01281
TGCAGCT3050.076.381551
GCCTGGT4400.075.087491
GCCTGGA2150.074.863611
ACTGGCT4800.074.124821
GCCTGGG2750.073.932291
CCAGGCT3400.073.501931
GGCAGCT2500.072.854111
GGCCCAA1400.072.6120761
GCCTGAT6750.070.281321
GCCTAGG2850.069.851971
ACCTGGG1850.068.6870961
GTCAGCT2900.068.647611
CTAGGCT3300.068.027981
GGCCTAG750.067.771271
CATGGCT2900.067.187031
CGCTGCT3000.064.947471
CCCTGGG2700.064.3199541
Produced by FastQC (version 0.11.5)