FastQCFastQC Report
Sat 7 Apr 2018
zr2096_6_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_6_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24237290
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT10500.078.102631
GCCTGAT9100.076.079871
GGCAGCT2450.075.69171
ACTGGCT4450.075.011331
CCAGGCT5500.074.927151
GGCCTAG550.074.927151
GCCTGGA3800.074.828551
GCCTGGG5400.074.788381
CTGTGCT3650.074.516591
GTCAGCT4600.074.357051
CTAGGCT3950.074.073541
CCGTGCT5650.073.6673051
GGCCCTA459.094947E-1173.26211
CCTGGCT7200.073.26211
GCCTGGT7800.072.9098741
TGCAGCT4750.072.008931
CAGTGCT3550.071.972271
CCCTGGG5800.071.7621151
GCCTAGG3000.071.4305341
GGCCGCT1050.070.645591
Produced by FastQC (version 0.11.5)