FastQCFastQC Report
Sat 7 Apr 2018
zr2096_7_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_7_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29534746
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT31150.080.954031
CAGTGCT12750.078.843181
CCGTGCT14750.077.763841
CTGTGCT16500.077.3269651
GTGTGCT22050.069.358521
GTCTGCT11150.069.351541
GGGTGCT11650.069.325081
GCATGCT2750.067.172921
CAGGGCT1100.066.391841
GCCTGGT3900.066.0914151
CCCTGCT37900.065.629311
CCTGGCT6050.065.326731
ACTGGCT4350.065.179811
CGCTGCT10950.065.12571
TGTGCTC71850.064.2364652
CCAGGCT4900.064.000781
GCCGGCT1450.062.2170941
GGCCTAT3200.061.754171
GGCCAAT2450.061.3706051
GCGTGCT11300.061.2076721
Produced by FastQC (version 0.11.5)