FastQCFastQC Report
Sat 7 Apr 2018
zr2096_8_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_8_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29761837
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCG357.9671736E-1085.019191
GCCTGCT16800.081.223691
CTGTGCT6050.080.100721
GCCTGGA3100.079.534081
CAGTGCT6100.078.7472841
GCCTGGT6350.077.655321
GCCTGGG4550.076.6106951
GTCAGCT5900.076.373171
CCTGGCT6300.076.247361
TGCAGCT5700.076.069791
CCGTGCT6350.075.646991
GCCTGAT7150.074.912011
CCAGGCT5600.074.391791
ACTGGCT5600.074.391791
GCTAGCT3550.073.0446551
CTAGGCT5200.072.7568051
GCCTAGG2650.072.1861041
GCCTGTA2550.071.6828461
GTCTGCT5650.071.476311
GGCCTAG555.456968E-1269.561161
Produced by FastQC (version 0.11.5)