FastQCFastQC Report
Sat 7 Apr 2018
zr2096_8_s1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_8_s1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29761837
Sequences flagged as poor quality0
Sequence length20-100
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCT10550.080.6002661
GCCTGCT25600.080.383021
CTGTGCT14200.078.1467741
CCGTGCT13600.077.8429641
GTCTGCT8700.075.748031
GCCTGGG2850.073.098781
GGCAGCT1850.071.24411
GGGTGCT11400.071.234021
GCCTAGG2050.070.51541
GTGTGCT18200.068.914341
CCTGGCT4350.068.417581
GCCTGGT4500.068.026631
TGTGCTC58550.066.5509642
GTCAGCT2950.066.297131
CCAGGCT4550.065.410221
GCCTGGA2750.064.93451
CGCTGCT9000.064.719781
GCGTGCT9350.064.5707241
CAGGGCT601.2732926E-1163.7749561
CCCTGCT30150.063.7397081
Produced by FastQC (version 0.11.5)