FastQCFastQC Report
Sat 7 Apr 2018
zr2096_9_s1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr2096_9_s1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32636231
Sequences flagged as poor quality0
Sequence length20-100
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGCT12100.078.543791
GCCTGCT19400.075.769131
GCCTGAT13950.072.669431
CCAGGCT6950.068.068781
CCTGGCT8850.067.296211
GCTGGCT7350.067.237771
CCGTGCT7950.066.41371
TGCAGCT7950.065.8823851
GGCTGCT8050.065.5886841
CCCAGCT15450.064.2471851
CCCTGCT21400.062.963911
CCCTGGT13350.062.9631651
TGTGCTC30250.061.85772
GCCTGAG8300.061.5774961
GGCAGCT3850.061.438711
CTGTGCT10200.060.874011
CCAGCTC58700.060.8042642
CGTGCTC20650.060.3416752
GCCTTAG7500.060.2611271
TCCTGGT12000.059.838741
Produced by FastQC (version 0.11.5)