Bismark report for: /Volumes/Serine/wd/18-04-07/zr2096_5_s1_R1.fastq.gz and /Volumes/Serine/wd/18-04-07/zr2096_5_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 31778715 Number of paired-end alignments with a unique best hit: 10617999 Mapping efficiency: 33.4% Sequence pairs with no alignments under any condition: 17366736 Sequence pairs did not map uniquely: 3793980 Sequence pairs which were discarded because genomic sequence could not be extracted: 3 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2702790 ((converted) top strand) GA/CT/CT: 2609389 (complementary to (converted) top strand) GA/CT/GA: 2601979 (complementary to (converted) bottom strand) CT/GA/GA: 2703838 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 366537981 Total methylated C's in CpG context: 34486919 Total methylated C's in CHG context: 2210810 Total methylated C's in CHH context: 6230765 Total methylated C's in Unknown context: 438615 Total unmethylated C's in CpG context: 13926155 Total unmethylated C's in CHG context: 82047506 Total unmethylated C's in CHH context: 227635826 Total unmethylated C's in Unknown context: 1526433 C methylated in CpG context: 71.2% C methylated in CHG context: 2.6% C methylated in CHH context: 2.7% C methylated in unknown context (CN or CHN): 22.3% Bismark completed in 0d 4h 34m 27s