Bismark report for: /Volumes/Serine/wd/18-04-10/sub-zr2096_5_s1_R1.fastq and /Volumes/Serine/wd/18-04-10/sub-zr2096_5_s1_R2.fastq (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-0.9 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 4325 Mapping efficiency: 43.2% Sequence pairs with no alignments under any condition: 4278 Sequence pairs did not map uniquely: 1397 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1109 ((converted) top strand) GA/CT/CT: 1062 (complementary to (converted) top strand) GA/CT/GA: 999 (complementary to (converted) bottom strand) CT/GA/GA: 1155 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 147077 Total methylated C's in CpG context: 13317 Total methylated C's in CHG context: 1034 Total methylated C's in CHH context: 3173 Total methylated C's in Unknown context: 338 Total unmethylated C's in CpG context: 5822 Total unmethylated C's in CHG context: 32564 Total unmethylated C's in CHH context: 91167 Total unmethylated C's in Unknown context: 1203 C methylated in CpG context: 69.6% C methylated in CHG context: 3.1% C methylated in CHH context: 3.4% C methylated in unknown context (CN or CHN): 21.9% Bismark completed in 0d 0h 0m 21s