Bismark report for: /Volumes/Serine/wd/18-04-10/10_10/sub-zr2096_5_s1_R1_val_1.fq and /Volumes/Serine/wd/18-04-10/10_10/sub-zr2096_5_s1_R2_val_2.fq (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 9896 Number of paired-end alignments with a unique best hit: 5200 Mapping efficiency: 52.5% Sequence pairs with no alignments under any condition: 2773 Sequence pairs did not map uniquely: 1923 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1345 ((converted) top strand) GA/CT/CT: 1242 (complementary to (converted) top strand) GA/CT/GA: 1224 (complementary to (converted) bottom strand) CT/GA/GA: 1389 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 136571 Total methylated C's in CpG context: 11867 Total methylated C's in CHG context: 798 Total methylated C's in CHH context: 4637 Total methylated C's in Unknown context: 401 Total unmethylated C's in CpG context: 5344 Total unmethylated C's in CHG context: 28548 Total unmethylated C's in CHH context: 85377 Total unmethylated C's in Unknown context: 1605 C methylated in CpG context: 69.0% C methylated in CHG context: 2.7% C methylated in CHH context: 5.2% C methylated in unknown context (CN or CHN): 20.0% Bismark completed in 0d 0h 0m 21s