FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4505002.8156250000000003TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3684582.3028625000000003TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2867721.7923250000000002TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2368261.4801625TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2274611.42163125TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2018201.261375TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC494170.30885625TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC392540.2453375TruSeq Adapter, Index 4 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC288150.18009375TruSeq Adapter, Index 5 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC270620.1691375TruSeq Adapter, Index 1 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC256560.16035000000000002TruSeq Adapter, Index 3 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC243180.1519875TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT241200.15075TruSeq Adapter, Index 7 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC234550.14659375TruSeq Adapter, Index 4 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC230690.14418124999999998TruSeq Adapter, Index 6 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT203380.1271125TruSeq Adapter, Index 4 (100% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC195020.1218875TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT175450.10965625000000001TruSeq Adapter, Index 5 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC173790.10861874999999999TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA330800.089.365691
CCGGAAG346500.088.886553
ACCGGAA358900.086.977652
ACGGAAG195200.079.645963
CATCGGA124650.078.3932341
GATTGGA211100.077.614261
ATTGGAA219700.076.915452
TGGAAGA259200.076.586374
TTGGAAG220600.076.441773
GAGCACA4214600.075.240469
GAACGGA197550.075.002361
AGAGCAC4246950.074.7492458
AAGAGCA4311800.073.9403467
GAAGAGC4317700.073.920726
GGAAGAG4424900.072.6251755
CGGAAGA4253500.071.378214
AACGGAA224550.069.794232
TCGGAAG3708750.069.6245353
ATCGGAA3796100.068.1995242
GATCGGA3764700.066.332691
Produced by FastQC (version 0.11.5)