FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5039793.1498687499999996TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3936002.46TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3186821.9917625TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2853261.7832875000000001TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2502541.5640874999999999TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2126431.32901875TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT306680.191675TruSeq Adapter, Index 7 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT230280.143925TruSeq Adapter, Index 4 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT216210.13513125TruSeq Adapter, Index 5 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT193210.12075625000000001TruSeq Adapter, Index 1 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC191480.11967499999999999TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC186930.11683125000000001TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT161430.10089375TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG151450.082.703853
GACCGGA138400.081.785371
ACCGGAA161100.078.401922
GAGCACA4023500.073.274769
CGAAGAG104350.072.918495
AGAGCAC4050000.072.8431248
AAGAGCA4120050.071.8937157
GAAGAGC4129550.071.8334666
TCCGAAG73850.071.549053
GGAAGAG4124800.070.2623445
TCGGAAG3731950.069.830143
GATTGGA138200.069.525341
CGGAAGA4028400.069.373984
ATCGGAA3812250.068.40942
CATCGGA76550.067.600921
ATTGGAA144800.067.4717252
GATCGGA3825200.066.8663941
TTGGAAG147200.066.0138553
TGGAAGA163400.065.046734
ACGGAAG86200.063.5582053
Produced by FastQC (version 0.11.5)