FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4287672.67979375TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3547052.21690625TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2753881.7211750000000001TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2229711.39356875TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2186811.36675625TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1967641.229775TruSeq Adapter, Index 6 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC356170.22260625TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC285580.1784875TruSeq Adapter, Index 4 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC274950.17184375TruSeq Adapter, Index 7 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC218840.136775TruSeq Adapter, Index 7 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC209120.1307TruSeq Adapter, Index 5 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC189190.11824375TruSeq Adapter, Index 1 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC186440.116525TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC179540.1122125TruSeq Adapter, Index 5 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC175240.10952500000000001TruSeq Adapter, Index 7 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC170990.10686875TruSeq Adapter, Index 4 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC167380.1046125TruSeq Adapter, Index 6 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT162270.10141874999999999TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG285400.087.87123
GACCGGA241950.087.236661
ACCGGAA297850.085.746442
CATCGGA228500.085.509231
TCTGAAG183450.078.842093
ACGGAAG156650.076.618583
CTGAAGA196200.076.572644
GAGCACA4338550.075.721139
GAAGAGC4393050.075.458976
AGAGCAC4369350.075.2916958
GCGGAAG111350.074.889013
AAGAGCA4436550.074.584957
AGCGGAA111950.074.530782
TGAAGAG201050.073.886585
GGAAGAG4344550.073.45935
GATTGGA167400.073.091671
CGGAAGA4213400.072.458914
TTGGAAG182100.072.0423053
TGGAAGA219400.071.7244
ATTGGAA183900.071.570372
Produced by FastQC (version 0.11.5)