FastQCFastQC Report
Tue 10 Apr 2018
2112_lane1_NoIndex_L001_R1_015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_NoIndex_L001_R1_015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881920
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC60910.690652213352685TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC53250.6037962626995645TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC38610.4377948113207547TruSeq Adapter, Index 5 (100% over 50bp)
TATCTGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC32890.372936320754717TruSeq Adapter, Index 7 (100% over 45bp)
TATCTGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC32150.3645455370101597TruSeq Adapter, Index 4 (100% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC32100.3639785921625544TruSeq Adapter, Index 1 (100% over 50bp)
TCTCTGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC31040.3519593613933236TruSeq Adapter, Index 7 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC30450.34526941219158197TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC29940.33948657474600874TruSeq Adapter, Index 6 (100% over 50bp)
TCTCTGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC29280.33200290275761973TruSeq Adapter, Index 4 (97% over 48bp)
TATCTGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC25280.2866473149492017TruSeq Adapter, Index 5 (100% over 45bp)
TCTCTGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC21750.24662100870827286TruSeq Adapter, Index 5 (97% over 48bp)
TATCTGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC17270.1958227503628447TruSeq Adapter, Index 3 (100% over 45bp)
TATCTGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC17050.19332819303338172TruSeq Adapter, Index 1 (100% over 45bp)
TATCTGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC16460.18663824383164004TruSeq Adapter, Index 6 (100% over 45bp)
TCTCTGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC15530.17609306966618288TruSeq Adapter, Index 3 (97% over 48bp)
TCTCTGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC15160.17189767779390422TruSeq Adapter, Index 1 (97% over 48bp)
TCTCTGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC14810.16792906386066764TruSeq Adapter, Index 6 (97% over 48bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC12520.14196298984034833TruSeq Adapter, Index 7 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC11820.13402576197387517TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT11510.1305107039187228TruSeq Adapter, Index 7 (100% over 50bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC10750.12189314223512338TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT10220.115883526850508TruSeq Adapter, Index 4 (100% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC9600.10885341074020319TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT9210.10443124092888244TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG79750.092.590515
TCTGAAG58950.092.394553
CTGAAGA80950.092.0978554
TCTCTGA23800.088.80221
CCTGAAG11650.086.412513
ATCTGAA33000.085.201742
TATCTGA26150.084.454331
CTCGGAA19350.084.434162
CTCTGAA28700.083.735442
GCTCGGA13000.082.2015151
TATCGGA8550.081.101231
GATCGCA5650.079.0169141
GCTCTGA3550.077.5961761
GCTGAAG7350.076.88233
GATCTGA6650.076.419261
GAGCACA196350.074.015939
AGAGCAC198300.073.383868
ACTGAAG7850.072.5902563
TCCCTGA5000.072.19121
AAGAGCA203400.072.150757
Produced by FastQC (version 0.11.5)