Processing paired-end Bismark output file(s) (SAM format): zr2096_6_s1_R1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>zr2096_6_s1_R1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_6_s1_R1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_6_s1_R1_bismark_bt2_pe.bam: 517 Total number duplicated alignments removed: 0 (0.00%) Duplicated alignments were found at: 0 different position(s) Total count of deduplicated leftover sequences: 517 (100.00% of total)