Bismark report for: /Volumes/Serine/wd/18-04-07/zr2096_6_s1_R1.fastq.gz and /Volumes/Serine/wd/18-04-07/zr2096_6_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000 Number of paired-end alignments with a unique best hit: 517 Mapping efficiency: 51.7% Sequence pairs with no alignments under any condition: 302 Sequence pairs did not map uniquely: 181 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 149 ((converted) top strand) GA/CT/CT: 107 (complementary to (converted) top strand) GA/CT/GA: 136 (complementary to (converted) bottom strand) CT/GA/GA: 125 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 16770 Total methylated C's in CpG context: 1717 Total methylated C's in CHG context: 112 Total methylated C's in CHH context: 449 Total methylated C's in Unknown context: 56 Total unmethylated C's in CpG context: 588 Total unmethylated C's in CHG context: 3620 Total unmethylated C's in CHH context: 10284 Total unmethylated C's in Unknown context: 239 C methylated in CpG context: 74.5% C methylated in CHG context: 3.0% C methylated in CHH context: 4.2% C methylated in unknown context (CN or CHN): 19.0% Bismark completed in 0d 0h 0m 15s