Processing paired-end Bismark output file(s) (SAM format): zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam If there are several alignments to a single position in the genome the first alignment will be chosen. Since the input files are not in any way sorted this is a near-enough random selection of reads. Checking file >>zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_10_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_10_s1_R1_val_1_bismark_bt2_pe.bam: 532162 Total number duplicated alignments removed: 1367 (0.26%) Duplicated alignments were found at: 1302 different position(s) Total count of deduplicated leftover sequences: 530795 (99.74% of total) Checking file >>zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_1_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_1_s1_R1_val_1_bismark_bt2_pe.bam: 288473 Total number duplicated alignments removed: 9143 (3.17%) Duplicated alignments were found at: 4833 different position(s) Total count of deduplicated leftover sequences: 279330 (96.83% of total) Checking file >>zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_2_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_2_s1_R1_val_1_bismark_bt2_pe.bam: 503476 Total number duplicated alignments removed: 5358 (1.06%) Duplicated alignments were found at: 3256 different position(s) Total count of deduplicated leftover sequences: 498118 (98.94% of total) Checking file >>zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_3_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_3_s1_R1_val_1_bismark_bt2_pe.bam: 526409 Total number duplicated alignments removed: 1361 (0.26%) Duplicated alignments were found at: 1266 different position(s) Total count of deduplicated leftover sequences: 525048 (99.74% of total) Checking file >>zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_4_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_4_s1_R1_val_1_bismark_bt2_pe.bam: 541137 Total number duplicated alignments removed: 783 (0.14%) Duplicated alignments were found at: 737 different position(s) Total count of deduplicated leftover sequences: 540354 (99.86% of total) Checking file >>zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_5_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_5_s1_R1_val_1_bismark_bt2_pe.bam: 520106 Total number duplicated alignments removed: 1247 (0.24%) Duplicated alignments were found at: 1147 different position(s) Total count of deduplicated leftover sequences: 518859 (99.76% of total) Checking file >>zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_6_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_6_s1_R1_val_1_bismark_bt2_pe.bam: 545007 Total number duplicated alignments removed: 820 (0.15%) Duplicated alignments were found at: 778 different position(s) Total count of deduplicated leftover sequences: 544187 (99.85% of total) Checking file >>zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_7_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_7_s1_R1_val_1_bismark_bt2_pe.bam: 518103 Total number duplicated alignments removed: 769 (0.15%) Duplicated alignments were found at: 751 different position(s) Total count of deduplicated leftover sequences: 517334 (99.85% of total) Checking file >>zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_8_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_8_s1_R1_val_1_bismark_bt2_pe.bam: 481891 Total number duplicated alignments removed: 2585 (0.54%) Duplicated alignments were found at: 1880 different position(s) Total count of deduplicated leftover sequences: 479306 (99.46% of total) Checking file >>zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam<< for signs of file truncation... samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 ...passed! Output file is: zr2096_9_s1_R1_val_1_bismark_bt2_pe.deduplicated.bam Total number of alignments analysed in zr2096_9_s1_R1_val_1_bismark_bt2_pe.bam: 506536 Total number duplicated alignments removed: 2728 (0.54%) Duplicated alignments were found at: 2521 different position(s) Total count of deduplicated leftover sequences: 503808 (99.46% of total) samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1