Bismark

Bismark Processing Report

zr2096_10_s1_R1_val_1.fq.gz and zr2096_10_s1_R2_val_2.fq.gz

Data processed at 13:52 on 2018-04-27


Alignment

Sequence pairs analysed in total 1,000,000
Paired-end alignments with a unique best hit 532,162
Pairs without alignments under any condition 258,809
Pairs that did not map uniquely 209,029
Genomic sequence context not extractable (edges of chromosomes) 0
Created with Highcharts 5.0.5: 53.2 %Unique AlignmentsUnique Alignments: 53.2 %: 25.9 %No AlignmentNo Alignment: 25.9 %: 20.9 %Multiple AlignmentsMultiple Alignments: 20.9 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 14,507,114
Methylated C's in CpG context 1,421,648
Methylated C's in CHG context 83,788
Methylated C's in CHH context 382,029
Methylated C's in Unknown context 37,110
Unmethylated C's in CpG context 604,367
Unmethylated C's in CHG context 3,125,846
Unmethylated C's in CHH context 8,889,436
Unmethylated C's in Unknown context 164,807
Percentage methylation (CpG context) 70.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 4.1%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 133,939 original top strand
CTOT 131,970 complementary to original top strand
CTOB 132,144 complementary to original bottom strand
OB 134,109 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB025k50k75k100k125k150k

Deduplication

Alignments analysed 532,162
Unique alignments 530,795
Duplicates removed 1,367
Duplicated alignments were found at 1302 different positions
Created with Highcharts 5.0.5: 99.7 %UniqueUnique: 99.7 %: 0.3 %DuplicateDuplicate: 0.3 %

Cytosine Methylation after Extraction

Total C's analysed 9,656,656
Methylated C's in CpG context 932,764
Methylated C's in CHG context 57,156
Methylated C's in CHH context 243,231
Unmethylated C's in CpG context 407,354
Unmethylated C's in CHG context 2,067,121
Unmethylated C's in CHH context 5,949,030
Percentage methylation (CpG context) 69.6%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 3.9%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls051015202530354045505560657075800102030405060708090100010k20k30k40k50k60k70k80k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls051015202530354045505560657075800102030405060708090100010k20k30k40k50k60k