Bismark report for: zr2096_10_s1_R1_val_1.fq.gz and zr2096_10_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000000 Number of paired-end alignments with a unique best hit: 532162 Mapping efficiency: 53.2% Sequence pairs with no alignments under any condition: 258809 Sequence pairs did not map uniquely: 209029 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 133939 ((converted) top strand) GA/CT/CT: 131970 (complementary to (converted) top strand) GA/CT/GA: 132144 (complementary to (converted) bottom strand) CT/GA/GA: 134109 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 14507114 Total methylated C's in CpG context: 1421648 Total methylated C's in CHG context: 83788 Total methylated C's in CHH context: 382029 Total methylated C's in Unknown context: 37110 Total unmethylated C's in CpG context: 604367 Total unmethylated C's in CHG context: 3125846 Total unmethylated C's in CHH context: 8889436 Total unmethylated C's in Unknown context: 164807 C methylated in CpG context: 70.2% C methylated in CHG context: 2.6% C methylated in CHH context: 4.1% C methylated in unknown context (CN or CHN): 18.4% Bismark completed in 0d 0h 10m 36s