Bismark

Bismark Processing Report

zr2096_1_s1_R1_val_1.fq.gz and zr2096_1_s1_R2_val_2.fq.gz

Data processed at 13:52 on 2018-04-27


Alignment

Sequence pairs analysed in total 1,000,000
Paired-end alignments with a unique best hit 288,474
Pairs without alignments under any condition 607,225
Pairs that did not map uniquely 104,301
Genomic sequence context not extractable (edges of chromosomes) 1
Created with Highcharts 5.0.5: 28.8 %Unique AlignmentsUnique Alignments: 28.8 %: 60.7 %No AlignmentNo Alignment: 60.7 %: 10.4 %Multiple AlignmentsMultiple Alignments: 10.4 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 7,000,478
Methylated C's in CpG context 585,279
Methylated C's in CHG context 53,657
Methylated C's in CHH context 541,383
Methylated C's in Unknown context 54,451
Unmethylated C's in CpG context 246,829
Unmethylated C's in CHG context 1,295,385
Unmethylated C's in CHH context 4,277,945
Unmethylated C's in Unknown context 118,630
Percentage methylation (CpG context) 70.3%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 11.2%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 72,189 original top strand
CTOT 73,122 complementary to original top strand
CTOB 72,138 complementary to original bottom strand
OB 71,024 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB020k40k60k80k

Deduplication

Alignments analysed 288,473
Unique alignments 279,330
Duplicates removed 9,143
Duplicated alignments were found at 4833 different positions
Created with Highcharts 5.0.5: 96.8 %UniqueUnique: 96.8 %: 3.2 %DuplicateDuplicate: 3.2 %

Cytosine Methylation after Extraction

Total C's analysed 4,534,552
Methylated C's in CpG context 385,582
Methylated C's in CHG context 32,257
Methylated C's in CHH context 275,567
Unmethylated C's in CpG context 164,917
Unmethylated C's in CHG context 864,563
Unmethylated C's in CHH context 2,811,666
Percentage methylation (CpG context) 70.0%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 8.9%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253035404550556065707580010203040506070809010005k10k15k20k25k30k35k40k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253035404550556065707580010203040506070809010002.5k5k7.5k10k12.5k15k17.5k20k22.5k25k27.5k