Bismark report for: zr2096_1_s1_R1_val_1.fq.gz and zr2096_1_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000000 Number of paired-end alignments with a unique best hit: 288474 Mapping efficiency: 28.8% Sequence pairs with no alignments under any condition: 607225 Sequence pairs did not map uniquely: 104301 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 72189 ((converted) top strand) GA/CT/CT: 73122 (complementary to (converted) top strand) GA/CT/GA: 72138 (complementary to (converted) bottom strand) CT/GA/GA: 71024 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 7000478 Total methylated C's in CpG context: 585279 Total methylated C's in CHG context: 53657 Total methylated C's in CHH context: 541383 Total methylated C's in Unknown context: 54451 Total unmethylated C's in CpG context: 246829 Total unmethylated C's in CHG context: 1295385 Total unmethylated C's in CHH context: 4277945 Total unmethylated C's in Unknown context: 118630 C methylated in CpG context: 70.3% C methylated in CHG context: 4.0% C methylated in CHH context: 11.2% C methylated in unknown context (CN or CHN): 31.5% Bismark completed in 0d 0h 6m 39s