Bismark

Bismark Processing Report

zr2096_2_s1_R1_val_1.fq.gz and zr2096_2_s1_R2_val_2.fq.gz

Data processed at 13:52 on 2018-04-27


Alignment

Sequence pairs analysed in total 1,000,000
Paired-end alignments with a unique best hit 503,477
Pairs without alignments under any condition 285,525
Pairs that did not map uniquely 210,998
Genomic sequence context not extractable (edges of chromosomes) 1
Created with Highcharts 5.0.5: 50.3 %Unique AlignmentsUnique Alignments: 50.3 %: 28.6 %No AlignmentNo Alignment: 28.6 %: 21.1 %Multiple AlignmentsMultiple Alignments: 21.1 %: 0.0 %No Genomic SequenceNo Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 12,425,132
Methylated C's in CpG context 1,171,780
Methylated C's in CHG context 75,002
Methylated C's in CHH context 557,179
Methylated C's in Unknown context 51,768
Unmethylated C's in CpG context 462,498
Unmethylated C's in CHG context 2,635,229
Unmethylated C's in CHH context 7,523,444
Unmethylated C's in Unknown context 169,378
Percentage methylation (CpG context) 71.7%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 6.9%
Methylated C's in Unknown context N/A%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 126,715 original top strand
CTOT 124,810 complementary to original top strand
CTOB 125,599 complementary to original bottom strand
OB 126,352 original bottom strand
Created with Highcharts 5.0.5Number of AlignmentsAlignment ContextOTCTOTCTOBOB025k50k75k100k125k150k

Deduplication

Alignments analysed 503,476
Unique alignments 498,118
Duplicates removed 5,358
Duplicated alignments were found at 3256 different positions
Created with Highcharts 5.0.5: 98.9 %UniqueUnique: 98.9 %: 1.1 %DuplicateDuplicate: 1.1 %

Cytosine Methylation after Extraction

Total C's analysed 7,583,893
Methylated C's in CpG context 710,832
Methylated C's in CHG context 45,981
Methylated C's in CHH context 303,174
Unmethylated C's in CpG context 287,332
Unmethylated C's in CHG context 1,613,757
Unmethylated C's in CHH context 4,622,817
Percentage methylation (CpG context) 71.2%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 6.2%
Created with Highcharts 5.0.5% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls051015202530354045505560657075800102030405060708090100010k20k30k40k50k60k70k
Created with Highcharts 5.0.5Position (bp)% Methylation# Methylation CallsRead 2Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls05101520253035404550556065707580010203040506070809010005k10k15k20k25k30k35k40k45k