Bismark report for: zr2096_3_s1_R1_val_1.fq.gz and zr2096_3_s1_R2_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/Serine/wd/18-03-15/genome/ with the specified options: -q --score-min L,0,-1.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000000 Number of paired-end alignments with a unique best hit: 526410 Mapping efficiency: 52.6% Sequence pairs with no alignments under any condition: 249675 Sequence pairs did not map uniquely: 223915 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 131363 ((converted) top strand) GA/CT/CT: 131574 (complementary to (converted) top strand) GA/CT/GA: 131909 (complementary to (converted) bottom strand) CT/GA/GA: 131563 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 14440042 Total methylated C's in CpG context: 1612313 Total methylated C's in CHG context: 77322 Total methylated C's in CHH context: 361714 Total methylated C's in Unknown context: 36630 Total unmethylated C's in CpG context: 469203 Total unmethylated C's in CHG context: 3140194 Total unmethylated C's in CHH context: 8779296 Total unmethylated C's in Unknown context: 172384 C methylated in CpG context: 77.5% C methylated in CHG context: 2.4% C methylated in CHH context: 4.0% C methylated in unknown context (CN or CHN): 17.5% Bismark completed in 0d 0h 10m 19s